- ￥6600
- GeneTex
- 美国
- GTX60370
- 2025年07月10日
- WB, ICC/IF, IP, IHC, RIP
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2).
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60370
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IP, IHC, RIP
- 浓度：
2.4 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
The antibody also recognizes other Ago family members although with the highest affinity for Ago2.
- 抗体英文名：
Argonautes antibody [GT9003]
- 抗体名：
Argonautes 抗体 [GT9003]
- 规格：
50 μg
IP analysis of HeLa cells (UV crosslinked and lysaed, and the the lysate was treated with DNase I) using GTX60370 Argonautes antibody [GT9003]. 10 μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded.
Antibody amount : 4 or 12μg.
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60370 Argonautes antibody [GT9003].
Red : Primary antibody
Blue : DAPI
Dilution : 1:500
WB analysis of whole cell extracts (40 μg) from HeLa cells using GTX60370 Argonautes antibody [GT9003].
Dilution : 1:500
RIP (RNA immunopreciptation) analysis of HeLa cells overexpressing cell lysate using GTX60370 Argonautes antibody [GT9003]. HeLa cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele).
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文献和实验Wang Z et al., EBioMedicine 2019 (PMID:30797711)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
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Antibody Screening of Bacteriophage gt11 DNA Expression Libraries
Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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Much of our current understanding of the molecular details of the activity and interactions of proteins has stemmed from the ability to isolate cDNA encoding these proteins. Computer-based analysis of deduced amino acid sequence allows
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