Recombinant human Ago2 protein (GST fusion protein containing amino acids 47–879 of Ago2).
亚型:
IgG1
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX60370
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, IP, IHC, RIP
浓度:
2.4 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
The antibody also recognizes other Ago family members although with the highest affinity for Ago2.
抗体英文名:
Argonautes antibody [GT9003]
抗体名:
Argonautes 抗体 [GT9003]
规格:
50 μg
IP analysis of HeLa cells (UV crosslinked and lysaed, and the the lysate was treated with DNase I) using GTX60370 Argonautes antibody [GT9003]. 10 μl of input (10% of starting material), 10 μl of supernatants after immunoprecipitation (10%), and all of immunoprecipitants (~90%) were loaded. Antibody amount : 4 or 12μg.
ICC/IF analysis of 4% PFA fixed HeLa cells using GTX60370 Argonautes antibody [GT9003]. Red : Primary antibody Blue : DAPI Dilution : 1:500
WB analysis of whole cell extracts (40 μg) from HeLa cells using GTX60370 Argonautes antibody [GT9003]. Dilution : 1:500
RIP (RNA immunopreciptation) analysis of HeLa cells overexpressing cell lysate using GTX60370 Argonautes antibody [GT9003]. HeLa cells were transfected with: pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1 (miR-1 is supposed to target “A” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-1C (miR-1C is supposed to target “G” allele) pRL-TK-4A + pRL-TK-4G + pcDNA-miR-377 (miR-377 is supposed to target neither allele).