- ￥5400
- GeneTex
- 美国
- GTX35255
- 2025年07月07日
- WB, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
COS cell-derived recombinant human IL-12, p40 subunit
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX35255
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ELISA
- 浓度：
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody is specific for natural IL-12 and recognizes the p70 heterodimer as well as the p40 subunit (monomers and homodimers). It will not cross-react with the p35 subunit.
- 抗体英文名：
IL12 antibody [C8.6] (Biotin)
- 抗体名：
IL12 抗体 [C8.6] (Biotin)
- 规格：
250 μg
Sandwich ELISA analysis of human IL12 protein in duplicate at 600, 240, 96, 38.4, 15.4, 0 pg/ml (50 ul per lane) using GTX79487 as capture antibody (7 ug/ml) and GTX35255 as detection antibody (0.03125 ug/ml). The plate was washed then incubated with Streptavidin-HRP. Detection was performed with TMB substrate. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
补体成分 C6/C7/C8/C9 补体成分：C6 血清浓度(μg/ml)：60 分子量(kDa)：128 亚单位(链)及分子量(kDa)：1条 生物学活性：组成MAC，触发淋巴细胞母细胞化 补体成分：C7 血清浓度(μg/ml)：60 分子量(kDa)：121 亚单位(链)及分子量(kDa)：1条
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