- ￥5300
- GeneTex
- 美国
- GTX53747
- 2025年07月14日
- WB, ELISA
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MYL4 (1-197aa) purified from E. coli
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX53747
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
MYL4
- 抗体英文名：
MYL4 antibody [AT4E8]
- 抗体名：
MYL4 抗体 [AT4E8]
- 规格：
100 μl
MYL4 antibody detects MYL4 protein by western blot analysis.
1. 40 μg HepG2 cell lysate
2. 40 μg K562 cell lysate
3. 40 μg MCF7 cell lysate
4. 40 μg Jurkat cell lysate
5. 40 μg 293T cell lysate
6. 40 μg LnCap cell lysate
MYL4 antibody (GTX53747) dilution: 1:1000
WB analysis of HeLa lysate (40ug) using MYL4 antibody at a dilution of 1:1,000.
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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
(placing the cells on an ice-water bath between pulses).To eliminate foaming,place the tip of the horn near the bottom of the tube while touching the side with the horn .USE THE PLAIN WHITE TUBES. 10.Centrifuge at maximal setting at4℃for 10-15 min. 11
marker somehow.Cut the top right-handcorner so that "up" is easily recognizable. 14)Wash with block 10-15 mins.on the rocker. 15)Transfer to the smallest container possible. Binding the antibody 16)Add diluted primary antibody. 17)Incubate primary
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