- ￥1700 - 4000
- GeneTex
- 美国
- GTX112477
- 2025年07月13日
- WB, IHC-P
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human alanyl-tRNA synthetase. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX112477
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P
- 浓度：
0.23 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alanyl-tRNA synthetase
- 抗体英文名：
alanyl-tRNA synthetase antibody [N3C2], Internal
- 抗体名：
alanyl-tRNA synthetase 抗体 [N3C2], Internal
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Sample (50 ug of whole cell lysate)
A: Mouse liver
5% SDS PAGE
GTX112477 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
D: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX112477 diluted at 1:1000
alanyl-tRNA synthetase antibody [N3C2], Internal detects AARS protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
alanyl-tRNA synthetase antibody [N3C2], Internal (GTX112477) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Halpert MM et al., FASEB J 2020 (PMID:32298026)
酶分类的主要类别之一。为催化与 ATP等三磷酸核苷的分解伴有形成新化学键可逆反应的酶之总称。催化具有有机化学意义的合成反应，分为两个分子相加的裂解酶和移换反应的移换酶两类。所以还是将不伴随腺苷三磷酸的分解而催化合成反应的酶称为合成酶（ Synthase）较为适宜。合成酶催化的反应含 ATP等基质，反应复杂， ATP分解为 ADP与正磷酸或 AMP与焦磷酸。根据生成键的种类而更分为：（ 1） C-O键，例如氨酰 tRNA合成酶；（ 2） C-S键，例如乙酰 CoA合成
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
技术资料暂无技术资料 索取技术资料
