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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C
- 库存:
大量
- 供应商:
欣博盛生物
- 抗体英文名:
REG III alpha antibody [C1C3]
- 抗体名:
REG III alpha antibody [C1C3]
- 规格:
25μl
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
, drying out will cause non-specific antibody binding and therefore high background staining. iii) Chymotrypsin can be very allergenic. Use a face mask and extraction cabinet for weighing out. iv) Prepare the chymotrypsin solution as quickly as possible
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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