- ￥1700 - 4000
- GeneTex
- 美国
- GTX108275
- 2025年07月12日
- WB
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human REG III alpha. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX108275
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
0.26 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
REG III alpha
- 抗体英文名：
REG III alpha antibody [C1C3]
- 抗体名：
REG III alpha 抗体 [C1C3]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with REG III alpha antibody [C1C3] (GTX108275) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot analysis of REG III alpha expression in transfected 293T cell line (20ug of whole cell lysate) A: Non-transfected lysate. B: REG III alpha transfected lysate. 12% SDS PAGE GTX108275 diluted at 1:500
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
, drying out will cause non-specific antibody binding and therefore high background staining. iii) Chymotrypsin can be very allergenic. Use a face mask and extraction cabinet for weighing out. iv) Prepare the chymotrypsin solution as quickly as possible
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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