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文献和实验Phosphoamino acid analysis:Mark Kamps's method
of PAA standards.15 min should be plenty. 13.Mark the plate with radioactive ink so that you can extrapolate where the origins were and can align the film unambigously with the plate and the PAA markers. 14.Expose the plate to flashed film with a screen
to precipitate as long as desired （up to overnight）。 To proceed, spin sample in a 4 !C microfuge at max speed for 30 minutes to 1 hour. 12. Aspirate off supernatant and wash pellet with 250 L 70% EtOH and spin down 5 . polyA RNA Isolation 13. Aspirate
Analysis of the Protein S Gene in Protein S Deficiency
Protein S (PS) is a 71-kDa vitamin K-dependent glycoprotein first identified in human plasma by DiScipio and colleagues in 1977 (1 ), a year after the discovery of the anticoagulant protein C (PC) (2 ,3 ). A few years later, Walker
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