相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Fibroblast Growth Factor 22 (FGF22)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Mus musculus (Mouse) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 19.9kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | His26~Ser162 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
HLEGD VRWRRLFSST HFFLRVDLGG RVQGTRWRHG QDSIVEIRSV RVGTVVIKAV YSGFYVAMNR RGRLYGSRVY SVDCRFRERI EENGYNTYAS RRWRHRGRPM FLALDSQGIP RQGRRTRRHQ LSTHFLPVLV SS
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验2008 Publisher: Springer-Verlag New York, LLC Series: Nucleic Acids and Molecular Biology Series , #22 ISBN-13: 9783540709374 ISBN: 3540709371
义翘神州日前曾邀请杨嘉慧博士进行了一场题为「获得高品质重组蛋白实验方案以及经典案例分析」技术公开课,课后收到了很多专业的提问,现将相关的问答简单列举一些,希望能够帮助更多的人获得高质量的重组蛋白。1. 是不是质粒片段越大转染效率越低?解答:一般情况是这样的,质粒片段越大,转染效率越低,但这也与表达宿主有关,不能一概而论。所以有时在进行表达载体优化时,会将质粒的片段或非重要功能区的一些片段进行删减,可以获得较好的转染效率。2.HEK293 瞬转表达重组蛋白相对于 CHO,哪个更有优势?解答
-HCl,1 mM EDTA,pH=8.0 注: 纯化所用所有试剂均用三蒸水进行定容,溶液配完后均用0.22 μm 滤膜经滤器过滤,4℃保存。 在实验中,可以先配制相应pH值的高浓度母液,在使用时 进行稀释。如: (1)1 M Tris-HCl,pH=8.0 称取60.57 g Tris溶于400 mL 三蒸水中,充分溶解,调pH至8.0,补加三蒸水定容至500 mL。用0.22 μm 滤膜过滤,置于玻璃瓶中4℃保存。 (2)2.5 M NaCl,pH=8.0 称取36.53 g
技术资料暂无技术资料 索取技术资料




