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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Adhesion Regulating Molecule 1 (ADRM1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 45.8kDa |
| Concentration | 5.6 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Thr2~Asp407 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
TTSGALFPS LVPGSRGASN KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFC WKDRTSGNVE DDLIIFPDDC EFKRVPQCPS GRVYVLKFKA GSKRLFFWMQ EPKTDQDEEH CRKVNEYLNN PPMPGALGAS GSSGHELSAL GGEGGLQSLL GNMSHSQLMQ LIGPAGLGGL GGLGALTGPG LASLLGSSGP PGSSSSSSSR SQSAAVTPSS TTSSTRATPA PSAPAAASAT SPSPAPSSGN GASTAASPTQ PIQLSDLQSI LATMNVPAGP AGGQQVDLAS VLTPEIMAPI LANADVQERL LPYLPSGESL PQTADEIQNT LTSPQFQQAL GMFSAALASG QLGPLMCQFG LPAEAVEAAN KGDVEAFAKA MQNNAKPEQK EGDTKDKKDE EEDMSLD
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验蛋白质的性质 方法 电荷(等电点) 离子交换(IEX) 分子量 凝胶过滤(GF) 疏水性 疏水
时间、在较高的菌密度下诱导都有利于包涵体的形成;在破菌和变性溶解时加入还原剂能够打开错配的二硫键;在复性液中加入二硫键异构酶、脯氨酸异构酶、分子伴侣和蛋白的辅助因子可以帮助重组蛋白折叠;精氨酸和高分子量PEG能减轻蛋白分子的聚集;尝试多种物理手段,如透析、快速稀释和柱上复性等,有时对复性有奇效。包涵体的表达相对简单,但其复性过程仍是依赖经验的,没有通用的方法可以套用。
重组菌的生长温度,降低培养温度是减少包涵体形成的最常用的方法,较低的生长温度降低了无活性聚集体形成的速率和疏水相互作用,从而可减少包涵体的形成[4]。 2、 添加可促进重组蛋白质可溶性表达的生长添加剂,培养E.coli时添加高浓度的多醇类、蔗糖或非代谢糖可以阻止分泌到周质的蛋白质聚集反应,在最适浓度范围内添加这些添加剂不会影响细胞的生长、蛋白质的合成或运输,其它促重组蛋白质可溶性表达的生长添加剂还有乙醇(诱导热休克蛋白的表达)、低分子量的巯基或二硫化合物(影响细胞周质的还原态,从而影响
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