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- 详细信息
- 技术资料
- 供应商:
研卉生物
- 检测范围:
0.781-50 ng/mL
- 检测方法:
ELISA
- 样本:
细胞裂解液, 组织提取液
- 规格:
96T
产品名称
Hsp70 StressXpress® ELISA试剂盒
货号
SKT-105
规格
96 孔板
Hsp70 StressXpress® ELISA试剂盒
货号
SKT-105
规格
96 孔板
- 产品类型
- ELISA
- 产品描述
- ELISA 试剂盒用来测定样本中 Hsp70 的浓度
- 研究领域
- 热休克
- 别名
- 热休克蛋白70, Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, HSPA1, HSPA1A, HSPA1B
- 适用物种
- 人
- 样本类型
- 细胞裂解液, 组织提取液
- 灵敏度
- 0.18ng/mL
- 最佳检测范围
- 0.781-50 ng/mL
- 培养时间
- 30 分钟
- 贮存温度
- 4℃
- 运输温度
-
蓝冰或4℃
货号 名称 数量/ 规格 SKC-105A Anti-Hsp70 Immunoassay Plate 1 Plate SKC-105B 5X Hsp70 Extraction Reagent 1 vial/10mL SKC-105C Recombinant Hsp70 Standard 2 vials SKC-105D Standard and Sample Diluent 1 vial/ 50mL SKC-105E 10X Wash Buffer Concentrate 1 vial/100mL SKC-105F Anti-Hsp70 Biotinylated Antibody Concentrate 1 vial/150µL SKC-105G Anti-Hsp70 Biotinylated Antibody Diluent 1 vial/ 13mL SKC-105H Streptavidin: HRP Concentrate 1 vial/150µL SKC-105I Streptavidin: HRP Diluent 1 vial/ 13mL SKC-105J TMB Substrate 1 vial/ 13mL SKC-105K Stop Solution 1 vial/ 13mL SKC-105L Pre-treatment Buffer 1 vial/ 13mL
- 参考文献
-
1. Zho, J. (1998) Cell. 94: 471-480.
2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38 (1) 1-17.
3. Rothman J. (1989) Cell 59: 591 -601.
4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887.
5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294.
6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
7. Smith D.F., et al., (1993) Mol. Cell. Biol. 13(2):869-876.
8. Prapapanich V., et al., (1996) Mol. Cell. Biol. 16(11):6200-6207.
9. Fernandez-Funez et al., (2000) Nature. 408(6808):101-106.
热休克蛋白背景知识
热休克蛋白 (HSPs) 存在于所有生命体的各种细胞里,功能也是多样的。 当细胞受到来自外环境的刺激时,例如高热,过冷,氧缺乏,它的表达就会增多,所以更多的时候它被认为是应激蛋白。
热休克蛋白不只是出现在细胞受到不利刺激的时候,它也在时刻监控着细胞的其他蛋白质,将他们由细胞中的一个区室(compartment)运到另一个区室, 或者是把衰老的蛋白运到指定地移除。热休克蛋白也被认为有帮助呈现特定氨基酸序列在细胞表面,从而使病态细胞易被免疫系统发现的功能。 热休克蛋白也有分子伴侣功能,确保细胞内的蛋白质以正确的形态出现在正确的时间,正确的地方。例如,热休克蛋白帮助新生成的或者扭曲的蛋白质折叠成正确的 形状以确保它们正常的功能。热休克蛋白主要有 Hsp90, Hsp70, Hsp60, Hsp40 and Hsp27,还有其他一些小分子热休克蛋白。
产品列表货号 产品名称 规格 种属 样本类型 灵敏度 检测范围 SKT-105-96 HSP70 ELISA Kit 96 well Human Cell Lysates | Tissue 0.18 ng/ml 0.781 - 50 ng/ml SKT-105-480 HSP70 ELISA Kit 5 x 96 well Human Cell Lysates | Tissue 0.18 ng/ml 0.781 - 50 ng/ml SKT-106-96 HSC70 ELISA kit 96 well Human Cell Lysates | Tissue | Serum 1.54 ng/ml 2.34 - 150 ng/ml SKT-106-480 HSC70 ELISA kit 5 x 96 well Human Cell Lysates | Tissue | Serum 1.54 ng/ml 2.34 - 150 ng/ml SKT-107-96 HSP90 Alpha ELISA kit 96 well Human Cell Lysates | Tissue | Serum 0.5 - 32 ng/ml SKT-107-480 HSP90 Alpha ELISA kit 5 x 96 well Human Cell Lysates | Tissue | Serum 0.5 - 32 ng/ml SKT-108-96 HSP70 ELISA Kit (High-Sensitivity) 96 well Human | Monkey |
Dog | Rat | MouseSerum | Plasma |
Cell Lysates | Tissue0.02 ng/ml 0.55 - 35 ng/ml SKT-108-480 HSP70 ELISA Kit (High-Sensitivity) 5 x 96 well Human | Monkey |
Dog | Rat | MouseSerum | Plasma |
Cell Lysates | Tissue0.02 ng/ml 0.55 - 35 ng/ml SKT-109-96 HSP27 ELISA Kit 96 well Human Serum | Plasma |
Cell Lysates | Tissue0.04 ng/ml 0.2 - 13 ng/ml SKT-109-480 HSP27 ELISA Kit 5 x 96 well Human Serum | Plasma |
Cell Lysates | Tissue0.04 ng/ml 0.2 - 13 ng/ml SKT-110-96 HSP60 ELISA Kit 96 well Human Cell Lysates | Tissue | Serum 1.37 ng/ml 7 - 450 ng/ml SKT-110-480 HSP60 ELISA Kit 5 x 96 well Human Cell Lysates | Tissue | Serum 1.37 ng/ml 7 - 450 ng/ml SKT-113-96 HSP65 ELISA Kit 96 well Human Cell Lysates | Tissue | Serum 0.06 ng/ml 0.344 - 22 ng/ml SKT-113-480 HSP65 ELISA Kit 5 x 96 well Human Cell Lysates | Tissue | Serum 0.06 ng/ml 0.344 - 22 ng/ml SKT-123-96 Alpha B Crystallin ELISA Kit 96 well Human Cell Lysates | Tissue | Serum 0.009 ng/ml 0.352 - 22.5 ng/ml SKT-123-480 Alpha B Crystallin ELISA Kit 5 x 96 well Human Cell Lysates | Tissue | Serum 0.009 ng/ml 0.352 - 22.5 ng/ml SKT-124-96 HSP70 ELISA Kit (PLANT) 96 well Plant | Human Plant extract | Cell Lysates |
Tissue | Serum | Plasma0.18 ng/ml 1.563 - 100 ng/ml SKT-124-480 HSP70 ELISA Kit (PLANT) 5 x 96 well Plant | Human Plant extract | Cell Lysates |
Tissue | Serum | Plasma0.18 ng/ml 1.563 - 100 ng/ml SKT-137-96 Rat GRP78 ELISA Kit 96 well Rat Tissue 0.15 ng/ml 0 - 50 ng/ml SKT-137-480 Rat GRP78 ELISA Kit 5 x 96 well Rat Tissue 0.15 ng/ml 0 - 50 ng/ml
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热休克蛋白检测试剂盒
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