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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
鼎国
- 库存:
大量
- 英文名:
Wilson protein nucleotide binding domain
- 规格:
5x0.5mg
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文献和实验Nucleotide Binding/Hydrolysis Assay
Nucleotide mix Motor (50 - 100 µM; purity > 95%) 0.5 M Tris-OAc, pH 7.5 10 mM EGTA 10 mM MgCl
Screening Peptide/Protein Libraries Fused to the Repressor DNA-Binding Domain in E. coli Cells
to detect protein-protein interactions in vivo. Protein or peptide targets are fused to the λ repressor DNA binding domain; these fusions can be evaluated for repressor activity using direct selection with λ phage, or a variety of reporter genes suitable
of a key amino acid for ligand binding and to determine the relationship between protein structure and ligand interaction. Here, we describe an application of this technique to evaluate different point mutations on the transcriptional activity of peroxisome
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