小鼠IgG1同型对照，低内毒素 | IgG1, Mouse,

Purification of Endotoxin Free mAbs

1) Endotoxin - you must assume that all standard laboratory glassware will be coated with loads of endotoxin. Wherever possible use sterile, specifically endotoxin-free (clinical grade) disposable plastics. If you need to use glassware or other materials

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

流式细胞术中为什么要用同型对照？如何选择同

择同型对照？ (1)一般选择与一抗的成分完全相同种属来源、相同亚型和亚链、相同荧光标记的抗体，比如抗人的CD56 FITC标记的抗体，成份是MouseIgG1,κ。那么它的同型对照应该是FITC标记的MouseIgG1,κ。注意同型对照的成份跟它的名称是相同的. (2)如果是抗体的组合形式是纯化的一抗＋荧光标记的二抗，那么应该选择一抗的同型对照。比如CD86的纯化抗体，成分是MouseIgG1,κ。它的同型对照是纯化的Mouse IgG1,κ。它的二抗PE标记的抗小鼠IgG1