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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Lecithin Cholesterol Acyltransferase (LCAT)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Homo sapiens (Human) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 23.0kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu41~His210 with two N-terminal Tags, His-tag and T7-tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
ELSNHTRPVI LVPGCLGNQL EAKLDKPDVV NWMCYRKTED FFTIWLDLNM FLPLGVDCWI DNTRVVYNRS SGLVSNAPGV QIRVPGFGKT YSVEYLDSSK LAGYLHTLVQ NLVNNGYVRD ETVRAAPYDW RLEPGQQEEY YRKLAGLVEE MHAAYGKPVF LIGHSLGCLH
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验卵磷脂胆固醇脂酰转移酶(lecithin-cholesterolacyltransferase)
卵磷脂胆固醇脂酰转移酶(lecithin-cholesterolacyltransferase,LCAT)由肝合成释放入血液,以游离或与脂蛋白结合的形式存在,是一种在血浆中起催化作用的酶,其作用是将HDL的卵磷脂的C2 位不饱和脂肪酸转移给游离胆固醇,生成溶血卵磷脂和胆固醇酯。血浆胆固醇几乎70%-80%是胆固醇酯,均是LCAT催化生成所致。LCAT常与HDL结合在一起,在HDL颗粒表面活性很高并起催化作用,对VLDL和LDL的颗粒几乎不起作用。LCAT在磷脂代谢中有重要
人卵磷脂胆固醇酰基转移酶(LCAT)酶联免疫分析(ELISA)
人卵磷脂胆固醇酰基转移酶(LCAT ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆, 组织及相关液体样本中卵磷脂胆固醇酰基转移酶(LCAT )的活性。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 卵磷脂胆固醇酰基转移酶(LCAT ) 水平。用纯化的人 卵磷脂胆固醇酰基转移酶(LCAT ) 抗体包被微孔板,制成固相抗体,往包
大鼠卵磷脂胆固醇酰基转移酶(LCAT ) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆, 组织及相关液体样本中卵磷脂胆固醇酰基转移酶(LCAT )的活性。 实验原理: 本试剂盒应用双抗体夹心法测定标本中大鼠 卵磷脂胆固醇酰基转移酶(LCAT ) 水平。用纯化的大鼠 卵磷脂胆固醇酰基转移酶(LCAT ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中
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