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文献和实验Blocking of unwanted non-specific staining
receptors). Crossreactivity of the secondary reagents with endogenous proteins. Blocking of endogenous enzymes Endogenous enzymes such as AlkPhos, AcPhos and esterases are destroyed by boiling Ref, even a short time at 100 C. Peroxidase
The Peroxidase-Antiperoxidase (PAP) Method
-antibody solution, the PAP immune complex. The horseradish peroxidase enzyme, itself an immunogenic protein, is used to inoculate a given species, and a polyclonal immune response is generated against the enzyme. This antiserum is harvested and placed
Western Blotting with Horseradish Peroxidase Conjugates
IN ALL STEPS USING HRPO (HORSERADISH PEROXIDASE) CONJUGATES. Dilute HRPO conjugated antibody in the corresponding blocking buffer Decant blocking buffer from the blot, add the diluted antibody solution and incubate with agitation for 30 minutes
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