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文献和实验Protein Syntheses in Cell Free Systems
. Refer to Appendix H for details on the use of a scintillation counter. Measure the amount of radioactively labeled amino acid incorporated into protein. Graph the protein synthesized versus time. Optional
proteinogenic/free amino acids or central carbon metabolites, and (2) a mathematical model that relates the unknown fluxes to the measured isotopomers. Modeling requires a thorough knowledge of the structure of the underlying metabolic network, often available
NOTE - Use arachidonic acid from Biomol (# FA-003) 1) Make up the appropriate concentration of AA in 100% ethanol. 2) Add 1µl of phenol red solution. --> The solution should appear yellow since the free acid
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