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文献和实验Screening BAC filters with non-radioactive probes
Science, Owashi 1-2, Tsukuba, Ibaraki 305-8634 , JAPAN) Probe labeling ( ECL Direct Nucleic Acid Labeling & Detection System. Amersham Biosciences Catalog #RPN3001 Take 500 ng DNA and 0.25 ng pBR322 in a Eppendorf tube
. If the membrane has been washed with water, a final wash step for 3 to 5 minutes in TBS and/or PBS containing 0.05% Tween 20 should follow. Perform the detection of proteins using detection system of choice, (e.g., enhanced chemiluminescence (ECL))
Studies of the Ubiquitin Proteasome System
Biomedicals Life Science Division, or see recipe ) or HRP‐conjugated anti–rabbit Ig and chemiluminescent detection reagents (SuperSignal West Pico, Pierce, or ECL Plus, GE Healthcare) Biomax MR film (Kodak
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