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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京鼎国昌盛
- 英文名：
0.5 ml Indivudual Tubes with attached Domed Cap
- 库存：
大量
- 规格：
1000
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文献和实验Lipoprotein Isolation - First week
ml plastic syringe (held by a clamp) attached to a 18 gauge needle and a capillary tubing (see picture ). 10. Let the hemolymph flow into the heat sealable centrifuge tube. 11. Add another 20 ml PBS into the syringe and overlay
Lipoprotein Isolation - First week
more (2-3 ml) PBS to the undissolved KBr and stir until dissolved. Pool these solutions and make up to 20 ml with PBS and stir further in the measuring cylinder. 9. Transfer solution into a 30 ml plastic syringe (held by a clamp) attached to a 18 gauge
Metabolic labeling Immunoprecipitation
) iii)IP 1.Prepare 1.7ml Eppendorf tubes (make sure that cap closes tightly) 2.Add 100-400µl of 35S-labeled culture sup or cell lysate 3.Add mAb (1µg for purified IgG,1µl for ascites,or 50µl for hybridoma supernatant) 4.Add 20µl of ProteinG-agarose
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