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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
96 lane sabertooth comb, 0.2 mm thick,
- 规格:
1
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文献和实验Analysis of ES Cell Clones by Mini-Southern
to solidify for about 30 minutes. This size of gel (3 lanes with 99 wells total) will accommodate one 96-well mini-Southern digest plate at 32 samples per lane plus one well per lane for molecular weight markers. 11. Remove the 96-well mini-Southern
ELECTROPHORESIS OF DNA IN AGAROSE GELS
plate or plastic tray surrounded by masking tape. Insert the comb parallel to the plate's edge, with the bottom of the teeth about 2mm above the plate. Multiple combs can be used on a gel to accommodate additional samples needing only short separation
Southern Blot Analysis(from Baker lab, university of Florida)
of plasmid DNA(a massive overkill).2.Allow restriction digest to proceed at 37℃ overnight.Electrophoresis1.Seal the gel casting platform at both ends with tape.Prepare and pour a100ml 0.8% agarose in 1X TBE.Insert the comb making sure there are no airbubbles
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