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- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京鼎国昌盛
- 英文名：
12 lane comb, adjustable height
- 库存：
大量
- 规格：
1
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文献和实验Southern Blot Analysis(from Baker lab, university of Florida
of Yeastchromosomal DNA per lane to be run. Digest 0.05 to 0.01μg of plasmid DNA(a massive overkill).2.Allow restriction digest to proceed at 37°C overnight. Electrophoresis 1.Seal the gel casting platform at both ends with tape. Prepare and pour a100mL
Southern Blot Analysis(from Baker lab, university of Florida)
DNA PrepPrepare DNA via your favorite method.You may find a protocol underMini Yeast Genomic Prep.Restriction Digest1.Digest DNA with appropriate restriction enzyme.Digest 10μg of Yeastchromosomal DNA per lane to be run.Digest 0.05 to 0.01μg
The ribonuclease protection assay (RPA)
12. Dilute the probe from Step 8 with hybridization buffer to the appropriate concentration: Add 2 µl of diluted probe to each RNA sample and mix by pipetting. Add a drop of mineral oil to each tube and quick spin in the microfuge. 13
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