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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
10 lane comb, 1.5 mm thick
- 规格:
2
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文献和实验Top 10 Fun Facts for DNA Electrophoresis
The minimum amount of DNA detectable by ethidium bromide on a 3-mm-thick gel and a 5-mm-wide lane is 1 ng. Do not exceed 50 ng of DNA per band on a 3-mm-thick gel and 5-mm-wide lane.
The ribonuclease protection assay (RPA)
% acrylamide 9.31 mls of 2% bis acrylamide 7.45 mls of 10x TBE 35.82 g of Urea QS to 74.5 ml with dH2O 450 µl ammonium persulfate (10%) 60 µl TEMED Pour immediately into the gel mold, remove any air bubbles, and add an appropriate comb (e.g
in gel tray about 1 inch from one end of the tray and position the comb vertically such that the teeth are about 1-2 mm above the surface of the tray Pour 50EC gel solution into tray to a depth of about 5 mm. Allow gel to solidify about 20 minutes
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