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- 文献和实验
- 技术资料
- 保存条件:
以说明书为准
- 保质期:
一年
- 英文名:
Antibiotic-Antimycotic Solution (100X)
- 库存:
大量
- 供应商:
欣博盛生物
- 规格:
1 x 100 ml
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文献和实验Preparation of Antibiotic Stock Solutions and Agar Plates
Antibiotic Stock SolutionsAmpicillinBeta-lactam-antibiotics are not very stable when dissolved. Slow but steady degradation happens even when frozen to -20°C. Therefore commercial beta-lactam-antibiotics are sold as dried powder and reconstituted
Preparing Antibiotics Stock Solution and Ampicillin Agar Plates
just before use! If possible adjust stock solution pH to stability optimum and choose suitable storage temperature! Due to enhanced solubility Ampicillin-Na is used for cell culture instead of Ampicillin. Optimal efficacy and stability for Ampicillin is at pH=4-5
Method: Removal of Yeast Contamination from Lymphoblast Cultures
only antibiotic and not the antimycotic. Solutions: Wash media: (1 liter) To 1 liter of sterile RPMI 1640 with 2mM L-glutamine, add: 10.0 ml 2.5 M (100X) Hepes buffer 1.2 ml 50 mg/ml gentamicin reagent Filter sterilize
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