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文献和实验A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
A general protocol for staining cell for cytometry analysis
Staining 1. Wash cells twice with cold PBS and then resuspend cells in 1X Binding Buffer at a concentration of ~1 x 10 6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture
In vitro transcription reaction
1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg) 7.8 ml DDW 5 ml 5X transcription buffer ( stratagene ) 1 ml 750 mM DTT 1 ml Rnasin (RNase inhibitor; 15U/ml) 1 ml 10 mM ATP (pH 7) 1 ml 10 mM CTP (pH
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