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文献和实验A general protocol for staining cell for cytometry analysis
2 µl PI and incubate for 15 min at RT. 5.Add 400 µl of 1× Binding Buffer to each tube. Analyze by flow cytometry as soon as possible (within 1 hr). Note: Methods for utilizing Annexin V binding on adherent cells ( i.e., monolayer
Intracellular Immunofluorescent Staining for Flow Cytometry
. No. 00-8222 ) eBioscience Permeabilization Buffer (Cat. No. 00-8333 ) eBioscience Flow Cytometry Staining Buffer (Cat. No. 00-4222 ) Instruments Pipettes and pipettors Centrifuge Ice bucket or refrigerator Flow
Intracellular Immunofluorescent Staining for Flow Cytometry
. No. 00-8222 ) eBioscience Permeabilization Buffer (Cat. No. 00-8333 ) eBioscience Flow Cytometry Staining Buffer (Cat. No. 00-4222 ) Instruments Pipettes and pipettors Centrifuge Ice bucket or refrigerator Flow
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