Genomic DNA Clean & Concentrat

HIGHLIGHTS

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• Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
• Unique spin column for low volume elution of ultra-pure, high-yield DNA
• Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.

DESCRIPTION

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The Genomic DNA Clean & Concentrator-25 (DCC) is a spin column gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.

DNA Recovery	Typically, up to 25 µg total DNA per column can be eluted into ≥35 µl of low salt DNA Elution Buffer or water.
DNA Size Limits	Capable of purifying small DNA fragments > 50 bp and large sized DNAs up to 200 kb.
Sample Sources	DNA from impure preparations of genomic DNA (e.g., Proteinase K digestions), plasmid DNA (including BAC), viral DNA, and whole genome amplified (wga) DNA. Can also be used for the purification of low molecular weight DNA (50 bp to 10 kb) from PCR, endonuclease digestion, post-RT cDNA synthesis, etc..
DNA Purity	High-quality (A(260/280) ≥ 1.8) high molecular weight DNA ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.
Product Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS.