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- 文献和实验
- 技术资料
- 保存条件：
干燥、避光
- 英文名：
1,2-O-Isopropylidene-beta-D-fructopyranose
- 库存：
10
- 供应商：
上海远慕生物
- 规格：
mg/g
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文献和实验References 1. R. M. O’Connell, K. D. Taganov, M. P. Boldin, G. Cheng, D. Baltimore, MicroRNA-155 is induced during the macrophage inflammatory response. Proc. Natl. Acad. Sci. U.S.A. 104, 1604–1609 (2007).[Abstract/Free Full Text] 2. D
Sucrose Density Gradient Fractionation
Grow a 2 ml culture,24 hr.at 30℃ in selective mediaWhen culture is ready,use it to inoculate about 55 ml (50 ml plus 5 for O.D.600 readings)of selective media.Grow to mid-logarithmic phase (O.D.600 is about 1).This usually takes 10 hours (for 1ml
β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
to an equal volume of Assay 2X Buffer, which contains the substrate ONPG (o-nitrophenyl-beta-D-galactopyranoside). Samples are incubated for 30 minutes, during which time the Beta-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl
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