Zymoclean Large Fragment DNA R

HIGHLIGHTS

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• Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels.
• Unique column design for low volume (≥ 10 µl) elution of ultra-pure, high-yield DNA.
• Eluted DNA is well-suited for use in endonuclease digestion, sequencing, labeling, PCR, etc.

DESCRIPTION

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The Zymoclean Large Fragment DNA Recovery Kit is a gel DNA extraction kit that provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.

Format	Spin Column
DNA Recovery	Typically, up to 10 μg total DNA per column can be eluted into ≥ 10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥ 23 kb, the recovery is 50-70%.
Equipment	Microcentrifuge
DNA Size Limits	50 bp to ~200 kb
Sample Sources	DNA excised from TAE and TBE-buffered agarose gels.
DNA Purity	High-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions.
Product Detergent Tolerance	≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS