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- 保存条件:
RT
- 英文名:
FastDNA® Spin Kit For Feces
- 库存:
大量
- 供应商:
武汉科昊佳
- 规格:
50次
产品名称:粪便DNA试剂盒FastDNA Spin Kit For Feces(MP)
产品规格:1 kit 50preps
产品货号:116570200
储存温度:15℃-30℃
产品描述,如下:
从人类和动物的粪便样品中有效分离基因组DNA,直接用于PCR
1.多达500mg的粪便样品
2.内含配合FastPrep仪器使用的理解介质E
3.通常可以从500mg的粪便样品中分离得到10-20μg总DNA
4.去除对下游实验有影响的
快速高效提取粪便DNA,可裂解任何粪便样品。可直接用于PCR等其下游实验,配合FastPrep仪器使用无需研磨样品。
The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.
Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
产品应用,如下:
The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
产品属性,如下:
| Product Type |
Kits & Assays |
| Components |
Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each |
| Instrument Compatibility |
FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1 |
| Sample Type |
Soil; Feces; Environmental Water; Wastewater; Sludge |
| Sample Volume |
up to 500 mg per prep |
| Tube Compatibility |
2 mL |
| Matrix Type |
E |
| Molecule Enriched |
DNA |
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文献和实验详情产品名基因组裂解方法纯化得率实验设备 BioTeke Soil Genomic DNA Rapid Extraction Kit 酶法破碎基因组完整特制纯化柱得率纯度高时间1小时内不需要特殊设备FastDNA® SPIN Kit钢珠破碎基因组断裂玻璃奶离心吸附柱纯化得率低时间2小时需要专门破碎设备UltraClean™ Soil DNA Isolation Kit玻璃珠破碎基因组断裂离心吸附柱纯化得率低时间2小时需要专门震荡器玻璃奶纯化得率60-75% ,离心吸附
℃下将收集的血浆分装保存,避免反复冻融。样本应避免溶血或高血脂。常见的抗凝剂包括EDTA钠盐,肝素钠,枸橼酸钠等。检测前请仔细阅读ELISA试剂盒说明书,查看该试剂盒针对抗凝剂是否有特殊要求。 细胞培养上清 将细胞培养上清液吸入离心管中,在2-8℃下以1000×g离心20分钟,除去细胞碎片和杂质,收集上清液备用,样本保存在-20℃或-80℃,避免反复冻融。 细胞提取液 反复冻融方法 1) 吸出培养板中的培养基,用胰蛋白酶消化细胞,然后加入适量的培养基将培养板上的细胞冲洗干净。悬浮
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于全市污水处理厂 (WWTP) 的每月腹泻病毒(诺如病毒基因组 I 和 II 以及轮状病毒)监测。通过 16 个月的临床监测,观察到废水中腹泻病毒浓度与粪便标本检出率之间存在显著相关性。废水中的诺如病毒浓度最高出现在冬季,这与香港诺如病毒爆发的季节性模式一致。此外,研究者们还建立了一种基于微小颗粒的方法来监测废水中的 DNA 病毒,并在香港首例 MPOX 患者入院时,在服务于约 16,700 人的污水处理厂的废水中检测到 MPOX 病毒的微弱信号。基因组测序为结果的有效性提供了确证证据。他们的研究
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