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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
HotStarTaq Master Mix Kit (250)
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文献和实验, temperatures, and amount of template DNA, may vary and must be individually determined. 1) Thaw primer solutions. Keep on ice after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important
should be visible after resuspension of the pellet. Add 250 μl Buffer P2 and gently invert the tube 4�6 times to mix. Mix gently by inverting the tube. Do not vortex, as this will result in shearing of genomic DNA. If necessary, continue inverting the tube
after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important to mix the PCR Master Mix before use to avoid localized differences in salt concentration. 3) Prepare one of the following reaction
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