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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
FastDNA® Spin Kit For Feces
- 库存:
大量
- 规格:
50preps
The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.
Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
产品应用,如下:
The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
产品属性,如下:
| Product Type | Kits & Assays |
| Components | Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each |
| Instrument Compatibility | FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1 |
| Sample Type | Soil; Feces; Environmental Water; Wastewater; Sludge |
| Sample Volume | up to 500 mg per prep |
| Tube Compatibility | 2 mL |
| Matrix Type | E |
| Molecule Enriched | DNA |
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文献和实验E.Z.N.A.® Endo-free Plasmid Mini Kit I Spin Protocol
strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5"® and JM109®. 2. Pellet 1.5-5 ml bacteria in appropriate vessels by centrifugation at 10,000 × g for 1 min at room temperature. 3. Decant or aspirate
Fastfilter Plasmid Maxi Kit Spin Protocol
h. For best results use overnight culture as the inoculum. It is strongly recommended that an endA negative strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5α® and JM109® . Optimal growth
Fastfilter Plasmid Midi Kit Spin Protocol
with agitation for 12-16 h. For best results use overnight culture as the inoculum. It is strongly recommended that an endA negative strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5α® and JM109® . Optimal
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