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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant HIV-p51/RT-p51(group M)Gag-Pol polyprotein Protein(His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
5.00 µg/20.00 µg/50.00 µg/100.00 µg
| 规格: | 5.00 µg | 产品价格: | ¥1010.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥1530.0 |
| 规格: | 50.00 µg | 产品价格: | ¥2830.0 |
| 规格: | 100.00 µg | 产品价格: | ¥4520.0 |
蛋白名称:HIV RT p51蛋白, HIV RT p51 protein
蛋白构建:A DNA sequence encoding the p51 (Reverse Transcriptase) of human immunodeficiency virus 1 gag-pol (NP_057849.4) (Pro588-Phe1027) was expressed with a polyhistidine tag at the N-terminus.
表达宿主:E. coli
蛋白纯度:> 90 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met
蛋白分子量:The recombinant p51 (Reverse Transcriptase) of human immunodeficiency virus gag-pol consists 458 amino acids and predicts a molecular mass of 53.6 kDa.
蛋白NP号:NP_057849.4
蛋白氨基酸序列:Pro588-Phe1027
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验LINEAGE ANALYSIS USING RETROVIRAL VECTORS
, these proteins must be supplied through complementation. The products of the genes, gag, pro, pol, and env are typically supplied by "packaging" cell lines or cotransfection with packaging constructs into highly transfectable cell lines (for review see Cepko
(GRAP) 177. Haemophila B Mutation Database IX http://www.umds.ac.uk/molgen/haemBdatabase.htm Point mutations, short additions and deletions in the Factor IX gene 178. HGVbase http://hgvbase.cgb.ki.se/ Curated human polymorphisms 179. HIV-RT
定性,据此可快速区别核酸靶的差异。例如检测序列5'GTATCAGCATXGCCATCGTGC中X碱基的种类,可用下列4种探针3'AGTCGTAACGGTAGC,3'AGTCGTACCGGTAGC,3'AGTCGTAGCGGTAGC,3'AGTCGTATCGGTAGC。高密度探针阵列可检则具有特征性的较长序列相关的多态性与变异。筛选1,000个核苷酸序列的变异与多态性需要4,000个探针。用100μm合成位点,设计1.28cm2阵列,将有大约16,000个探针,能筛选4kb序列。HIV-1基因组中rt与pro
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