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100 µg
蛋白名称:PSPH蛋白, PSPH protein
蛋白构建:A DNA sequence encoding the human PSPH (P78330) (Met 1-Glu 225) was expressed and purified.
表达宿主:E. coli
蛋白纯度:> 84 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met 1
蛋白分子量:The recombinant human PSPH consists of 225 amino acids and has a calculated molecular mass of 25KD. It migrates as an approximately 28KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P78330
蛋白氨基酸序列:Met1-Glu225
蛋白标签:Native
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Purification of acidic phosphatase from mustard seedlings
an artificial substrate that changes its color after hydrolytic removal of the phosphate group: Since the phosphatase is active only at acidic pH values, but p-nitrophenol is colored at basic pH values, we must change the pH following the enzyme
Purification of acidic phosphatase from mustard seedlings
Purification of acidic phosphatase from mustard seedlings Phosphate esters are widely distributed in any organism. Nucleic acids, metabolic intermediates like glucose-6-phosphate, energy-rich substrates (AMP, creatine phosphate
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
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