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50 µg
蛋白名称:p67phox蛋白, p67phox protein
蛋白构建:A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human NCF2 /GST chimera consists of 763 amino acids and has a calculated molecular mass of 87.6 kDa. The recombinant protein migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:AAH01606.1
蛋白氨基酸序列:Met1-Val526
蛋白标签:N-GST & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells
subunits. Equimolar amounts of p47phox and p67phox in the cytosol combine to a form a highly basic 250 kDa protein. During activation, this complex migrates from the cytosol to the membrane where this complex combines with cytochrome b558 , which in turn
Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation
also participates in activation of the nonphagocytic oxidase Nox1; in this case, GTP-bound Rac functions by interacting with Noxa1, a p67 phox -related protein that is required for Nox1 activation. On the other hand, in the presence of either p67 phox or Noxa
Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS
of the family of Rho-like proteins. Rat effectors include the p21-activated kinases (PAK) ( 4 , 5 ), and the p67-phox of nicotrnamide adenine dinueleotide phosphate (reduced form) (NADPH) oxrdase ( 6 ). CDC42Hs effecters include PAK kinases and the Wiskott
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