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- Sino Biological
- 50728-MNCB
- 2025年08月12日
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- 规格：
100 µg
蛋白名称：Smad5蛋白, Smad5 protein
蛋白构建：A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly
蛋白分子量：The recombinant mouse SMAD5 consists of 466 amino acids and predicts a molecular mass of 52.2 KDa. It migrates as an approximately 57 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P97454
蛋白氨基酸序列：Thr2-Ser465
蛋白标签：N-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验At the onset of mammalian kidney development, the ureteric bud invades the surrounding metanephric mesenchyme, and genetic studies in the mouse have shown that BMP pathway activity has to be antagonized in the vicinity of the epithelium
James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
Thrombin Cleavage of GST-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of thrombin, temperature and length of incubation must be calibrated
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