- ￥2310 - 4520
- Sino Biological
- 北京
- 80545-R08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Rat Cathepsin B / CTSB Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/50.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥4520.0 |
蛋白名称：Rat Cathepsin B / CTSB Protein (His Tag)
蛋白构建：A DNA sequence encoding the rat Ctsb (NP_072119.2) (Met1-Phe339) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：His 18
蛋白分子量：The recombinant rat Ctsb consists 333 amino acids and predicts a molecular mass of 37.1 kDa.
蛋白NP号：NP_072119.2
蛋白氨基酸序列：Met1-Phe339
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Small Scale GST-Tag Purification Under Nature Conditions
of overloading). 3) Wash beads with 2x1.5ml PBS (washing: mix, spin 3min 3500rpm, keep supernatant aside). Keep sample of 40ul for PAGE-SDS of each washing . 4) Elute recombinant protein with 3 x 50ul elution buffer: 50mM TrisHCl pH8.0 10mM reduce
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
) was exchanged with the multiple cloning site of pET15b (sequences between the Xba I to Bpu 1102I sites). The HMK coding sequence was then inserted by recombinant PCR directly downstream of and in frame with the His tag
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