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100 µg
蛋白名称：E-Selectin蛋白, E-Selectin protein
蛋白构建：A DNA sequence encoding the cynomolgus SELE (EHH61734.1) (Met1-Pro556) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE.
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to Cynomolgus/Rhesus SELP/Fc Chimera coated plates (2 μg/mL, 100 μL/well), > 40% cells will adhere after 1 hour at 37℃.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Trp 22
蛋白分子量：The recombinant cynomolgus SELE consists 776 amino acids and predicts a molecular mass of 85.7 kDa.
蛋白NP号：EHH61734.1
蛋白氨基酸序列：Met1-Pro556
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验人E-选择素(E-Selectin)ELISA试剂盒 说明书
人E- 选择素 (E-Selectin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E-Selectin 单抗包被于酶标板上，标准品和样品中的 E-Selectin与单抗结合，加入生物素化的抗人 E-Selectin ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物
-like domain),约含35个氨基酸残基，EGF样结构域虽不直接参加配体的结合，但对维持selectin分子的构型是必需的；（3）近胞膜部分是数个由约60个氨基酸残基构成的补体调节蛋白（complement regulatory protein)重复序列或称为补体结合蛋白（complement binding protein)重复序列，它们与补体受体（如CR1、CR2等）和C4结合蛋白（C4bp)等结构同源。各种selectin分子的穿膜区和胞浆区没有同源性（见图2-4）。selectin分子的胞浆区与细胞
Membrane Protein Insertion in E. coli
, the insertion of proteins into the membrane is catalyzed by the Sec translocase and the YidC membrane insertase. YidC can function on its own to insert proteins or together with the Sec translocase to facilitate membrane protein insertion. In this chapter
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