Interferon alpha 2 / IFNA2 蛋白|Interferon alpha 2 / IFNA2 protein|Interferon alpha 2 / IFNA2(Rhesus, His Tag)
- ￥3480
- Sino Biological
- 90105-C08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
100 µg
蛋白名称：Interferon alpha 2/IFNA2蛋白, Interferon alpha 2/IFNA2 protein
蛋白构建：A DNA sequence encoding the rhesus IFNA2 (NP_001129266.1) (Met1-Glu188) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.4-4 pg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Rhesus IFNA2-His (cat:90105-C08H) at 10 μg/ml (100 μl/well) can bind human IFNAR2-Fc (Cat:10359-H02H) with a linear range of 0.31-2.5 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Cys 24
蛋白分子量：The recombinant rhesus IFNA2 comprises 176 amino acids and has a calculated molecular mass of 21 KDa.
蛋白NP号：NP_001129266.1
蛋白氨基酸序列：Met1-Glu188
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
of phosphorylation sites in proteins: Construction of a phosphorylatable human interferon alpha. Proc. Natl. Acad. Sci. U.S.A. 86:558‐562. Loizos, N. and Darst, S.A. 1998. Mapping protein
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
-19]； xChIP：用来研究DNA及低结合力蛋白，采用甲醛或紫外线进行 DNA 和蛋白交联，超声波片段化染色质，然后进行片段富集及后续分析，适用于多数非组蛋白的蛋白，例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后，对 DNA 进行 PCR 扩增，验证目标序列的存在。除验证实验外，ChIP DNA也可以进行测序分析，这种方法被称为 ChIP-seq [22] ；也可做芯片分析，这种方法被称为 ChIP on CHIP 或 ChIP
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