- ￥580 - 4980
- Sino Biological
- 北京
- 50708-MCCH
- 2026年01月26日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse IFNB1 / IFN-beta / Interferon beta Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
5.00 µg/20.00 µg/100.00 µg
| 规格： | 5.00 µg | 产品价格： | ￥580.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥2310.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4980.0 |
蛋白名称：IFN-beta蛋白, IFN-beta protein
蛋白构建：A DNA sequence encoding the mouse IFNB1 (P01575) (Met 1-Asn 182) was fused with five amino acids (DDDDK) at the C-terminus was expressed and purified.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 3-18 pg/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Val 19
蛋白分子量：The recombinant mouse IFNB1 consists of 166 amino acids and has a calculated molecular mass of 20.3 kDa. The recombinant protein migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P01575
蛋白氨基酸序列：Met1-Asn182
蛋白标签：C-cleavage
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Wang, ZW; et al. Repurposing of the FGFR inhibitor AZD4547 as a potent inhibitor of necroptosis by selectively targeting RIPK1. Acta pharmacologica Sinica, PMID: 36216899
3, Ren, X; et al. Nucleic DHX9 cooperates with STAT1 to transcribe interferon-stimulated genes. Science advances, PMID: 36735791
4, Qin, Y; et al. Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation. The Journal of clinical investigation, PMID: 37651190
5, Zhao, C; et al. Polyamine metabolism controls B-to-Z DNA transition to orchestrate DNA sensor cGAS activity. Immunity, PMID: 37848037
6, Hou, S; et al. PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis. Molecular cell, PMID: 38272024
7, Shi, Y; et al. Acquired resistance to PD-L1 inhibition enhances a type I IFN-regulated secretory program in tumors. EMBO reports, PMID: 39663510
8, Yang, ZH; et al. ZBP1 senses splicing aberration through Z-RNA to promote cell death. Molecular cell, PMID: 40267920
9, Qiao, L; et al. Optogenetic-Controlled iPSC-Based Vaccines for Prophylactic and Therapeutic Tumor Suppression in Mice. Advanced science (Weinheim, Baden-Wurttemberg, Germany), PMID: 40619603
10, Lei, H; et al. Stress granule-mediated ZBP1 activation drives necroptotic cell death in non-obstructive azoospermia and testicular aging. Proceedings of the National Academy of Sciences of the United States of America, PMID: 40811463
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
Beta-gal Assay Adapted from Current Protocols in Molecular Biology 1. Grow 5ml YEPD cultures to mid-log phase. 2. Centrifuge and resuspend cells in 5ml Z-buffer, then place on ice. 3. Measure OD600. 4. Use straight
. Here we detail a collection of techniques that include fluorescent in vivo imaging, X/Y FISH, and beta-galactosidase detection that are used for following labeled cells in vivo after adoptive transfer or transplant of donor cells for identifying the migration and engraftment
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