- ￥2310 - 3870
- Sino Biological
- 北京
- 80350-R08H
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Rat ADAM17 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/20.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：ADAM17蛋白, ADAM17 protein
蛋白构建：A DNA sequence encoding the rat ADAM17 (Q9Z1K9) (Met1-Asp563) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003). The specific activity is >250 pmol/min/µg.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Pro 18
蛋白分子量：The recombinant rat ADAM17 comprises 557 amino acids and predicts a molecular mass of 63 kDa. The apparent molecular mass of the recombinant protein is approximately 57 and 20 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：Q9Z1K9
蛋白氨基酸序列：Met1-Asp563
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
are produced as soluble recombinant proteins by transiently transfecting a mammalian cell line. The proteins are purified using an oligo‐His tag using a bespoke purification apparatus (the “Protein Press”) and subsequently printed onto glass slides
a protein to be overexpressed are fused to a fluorescent protein or an epitope-tag, plasmid electrotransfer permits visualization of the expressed protein in muscle fibers. Here, we demonstrate the use of electrotransfer of plasmids encoding mutant or wild
Strategies to Optimize Protein Expression in E. coli
Abstract Table of Contents Figures Literature Cited Abstract Recombinant protein expression
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