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- Sino Biological
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- 2025年08月12日
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- 规格：
50 µg
蛋白名称：TWEAK/TNFSF12蛋白, TWEAK/TNFSF12 protein
蛋白构建：A DNA sequence encoding the rat TNFSF12 (Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 5-20 ng/mL. 2. Immobilized Fc-rat TNFSF12 at 10 μg/ml (100 μl/well) can bind biotinylated human Fc-TNFRSF12A (Cat:10431-H01H), The EC50 of biotinylated human Fc-TNFRSF12A (Cat:10431-H01H) is 0.12-0.28 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu
蛋白分子量：The recombinant rat TNFSF12 comprises 405 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：Q6AYC1_RAT
蛋白氨基酸序列：Arg105-His249
蛋白标签：N-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
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