Recombinant Human CALR / Calreticulin Protein (His Tag) | 重组人 CALR / Calreticulin 蛋白 (His标签)
- ￥3220 - 25190
- Sino Biological
- 北京
- 13539-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CALR / Calreticulin Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg/500 µg
| 规格： | 50 µg | 产品价格： | ￥3220.0 |
|---|---|---|---|
| 规格： | 500 µg | 产品价格： | ￥25190.0 |
蛋白名称：Human CALR / Calreticulin Protein (His Tag)
蛋白构建：A DNA sequence encoding the human CALR (P27797) (Met1-Ala413) was expressed with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 18
蛋白分子量：The recombinant human CALR consists of 407 amino acids and predicts a molecular mass of 47.4 KDa. It migrates as an approximately 58 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P27797
蛋白氨基酸序列：Met1-Ala413
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
functional biology and proteomics. Mol. Biochem. Parasitol. 118:155‐165. Bussow, K., Nordhoff, E., Lubbert, C., Lehrach, H., and Walter, G. 2000. A human cDNA library for high
Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Small Scale GST-Tag Purification Under Nature Conditions
4°C. d) ßME, DTT or DTE up to 10mM for proteins with many cysteines. e) 0.1-2% Triton X-100, NP40; or any other detergent that do not affect the biological activity of your protein. Sonicate in ice bucket 3 x 10sec or more if the cells
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