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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Rat VEGF-D / VEGFD / FIGF Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/50.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥1580.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥2980.0 |
蛋白名称:VEGFD蛋白, VEGFD protein
蛋白构建:A DNA sequence encoding the rat FIGF (Phe94-Arg210) was expressed, fused with the Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized recombinant Rat VEGF-Dprotein (Cat:80104-R01H) at 10 µg/mL (100 µl/well) can bind biotinylated mouse VEGFR3-Fc (Cat:50584-M02H) with a linear range of 31.25-2000 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu
蛋白分子量:The recombinant Rat FIGF/Fc comprises 377 amino acids and has a predicted molecular mass of 41.6 kDa. The apparent molecular mass of the protein is approximately 52 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:VEGFD_RAT
蛋白氨基酸序列:Phe94-Arg210
蛋白标签:N-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
可能与其他物种发生交叉反应, 除非特异性的经过交叉反应物种的吸附处理。在抗体描述中,经过其他物种的吸附处理的抗体标有“ (min X …Sr Prot) ”字样 。 Anti-IgG (H+L) 这类抗体和 IgG 分子的重链和轻链都可以反应,比如,和 IgG 分子的 Fc , F(ab ’)2/Fab 部分。 Anti-IgG (H+L) 抗体也可以和其他的免疫球蛋白家族( IgM , IgA ,IgD,IgE )和亚家族反应,因为所有的免疫球蛋白都有相同的轻链(或者 κ 链
in recombinant Escherichia coli, Biochem. J. 358 (2001) 263-268. 35. G. Lemercier, N. Bakalara, X. Santarelli, On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli, J
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