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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Rat IL-1RA / IL1RN Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:IL1RA蛋白, IL1RA protein
蛋白构建:A DNA sequence encoding the rat IL1RN (P25086) (His27-Gln178) was expressed, fused with Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Immobilized rat IL1R1-His (Cat:80028-R08H) at 10 μg/ml (100 μl/well) can bind rat Fc-IL1RN, The EC50 of rat Fc-IL1RN is 5.1-11.9 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu
蛋白分子量:The recombinant rat IL1RN comprises 412 amino acids and predicts a molecular mass of 45.8 kDa. The apparent molecular mass of the recombinant protein is approximately 47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:P25086
蛋白氨基酸序列:His27-Gln178
蛋白标签:N-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Zhan L, et al. Inhibition of MLKL-dependent necroptosis via downregulating interleukin-1R1 contributes to neuroprotection of hypoxic preconditioning in transient global cerebral ischemic rats.Journal of neuroinflammation, PubMed ID: 33879157
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
IgG, 这种抗体在检测亚美尼亚仓鼠IgG 单克隆抗体时,它的效果没有抗亚美尼亚仓鼠IgG 好。 注意:抗亚美尼亚仓鼠IgG(H+L)(min X Bov, Hu, Ms , Rb, Rat Sr Prot) 不能识别所有的亚美尼亚仓鼠单克隆抗体,因为它经相近物种的吸附处理过(粗体字)。因此,使用经过少数物种或少数相近物种的吸附处理过的抗体可达到较好的效果,如抗亚美尼亚仓鼠IgG(H+L)(min X BoV Sr Prot), 除此之外,在小鼠或大鼠免疫球蛋白中也应该检测亚美尼亚仓鼠单抗。
15226268 REMARK GeneRIF: HO-1 interacts with caveolin-1 (inhibitory) and localizes in part to specialized plasma membrane caveolae in rat pulmonary artery endothelial cells. REFERENCE 12 (bases 1 to 1600) AUTHORS Huang,X.L., Ling,Y.L., Ling,Y.Q., Zhou
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