- ￥1080 - 22680
- Sino Biological
- 北京
- 51113-M02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse FLT3L / Flt3 ligand Protein (Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/10.00 µg/20.00 µg/100.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥22680.0 |
|---|---|---|---|
| 规格： | 10.00 µg | 产品价格： | ￥1080.0 |
| 规格： | 20.00 µg | 产品价格： | ￥1920.0 |
| 规格： | 100.00 µg | 产品价格： | ￥3480.0 |
蛋白名称：Flt3 Ligand蛋白, Flt3 Ligand protein
蛋白构建：A DNA sequence encoding the mouse FLT3L (P49772-1) (Met1-Arg188) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured in a cell proliferation assay using BaF3 mouse proB cells transfected with mouse Flt3. The ED50 for this effect is typically 7-30 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly 27
蛋白分子量：The recombinant mouse FLT3L /Fc is a disulfide-linked homodimer. The reduced monomer comprises 403 amino acids and has a predicted molecular mass of 45.4 kDa. The apparent molecular mass of the protein is approximately 56 and 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：P49772-1
蛋白氨基酸序列：Met1-Arg188
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
represent the migratory and inflammatory DC subtypes and not the DC subtypes found in the steady state. By contrast a different culture method was described where mouse bone marrow is cultured with flt3 ligand for 9 days. Here, we describe this method
【求助】新手 请问western-blot测NF-KB、FLT3是用总蛋白提取试剂盒吗
-3, extracellular region; 200 µg /Anti-Flt-3, cytoplasmic domain; 200 µg /Flt-3 Ligand, recombinant human; 10 µg 。不知道用那一种好！可惜我是搞临床的，免疫和分子生物学很差，知道不多，问了该公司技术支持，也没说出什么，建议查参考文献。东查西查也没有弄清？急盼各位高手给答案。万分感谢！ lwjssry 看了你描述的三种抗体，推测flt3是一种膜受体吧，不知猜得对不对？
The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
immunofluorescent labeling and flow cytometric cell sorting. If the availability of mice is limiting, our protocol can cater for DC numbers boosted by the administration of fms-like tyrosine kinase 3 ligand (Flt3L), directly via subcutaneous injection
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