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100 µg
蛋白名称:IL21 Receptor蛋白, IL21 Receptor protein
蛋白构建:A DNA sequence encoding the rat IL21R (Q5EBB1) (Met1-Pro236) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Immobilized rat IL21R-His at 10 μg/ml (100 μl/well) can bind biotinylated rat IL21-His (Cat:80200-R08B), The EC50 of biotinylated rat IL21-His (Cat:80200-R08B) is 4.6-10.9 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Cys 20
蛋白分子量:The recombinant rat IL21R comprises 228 amino acids and predicts a molecular mass of 26.5 kDa. The apparent molecular mass of the recombinant protein is approximately 43-48 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:Q5EBB1
蛋白氨基酸序列:Met1-Pro236
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
in detail. The process of fusing the two components, the receptor and the G-protein α-subunit, result in a construct that acts as an agonist stimulated GTPase enzyme whose expression level can be accurately determined by radioligand binding. The effects of different
G Protein-Coupled Receptor Pharmacogenetics
Common G protein-coupled receptor (GPCR) gene variants that encode receptor proteins with a distinct sequence may alter drug efficacy without always resulting in a disease phenotype. GPCR genetic loci harbor numerous variants, such as DNA
Receptor Isolation and Characterization: From Protein to Gene
capture the biomolecule of interest. Isolation of a protein by affinity chromatography is a very effective technique. It provides the protein in a pure state, enabling its identification by partial sequencing, either by mass spectrometry or by N-terminal
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