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20 µg
蛋白名称：TIMP-1蛋白, TIMP-1 protein
蛋白构建：A DNA sequence encoding the rat TIMP1 (P01860) (Met1-Ala217) was expressed and purified.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate MCA-PLGLDPA-AR-NH2, AnaSpec Catalog # 27076. The IC50 value is approximately 4 nM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Cys 24
蛋白分子量：The recombinant rat TIMP1 consists of 194 amino acids and has a predicted molecular mass of 21.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：P01860
蛋白氨基酸序列：Met1-Ala217
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠TIMP-2 ( TIMP-2 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 TIMP-2 单抗包被于酶标板上，标准品和样品中的 TIMP-2与单抗结合，加入生物
Analysis of TIMP Expression and Activity
This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence
Mouse Models of MMP and TIMP Function
-deficient and TIMP-null mice and strategies and pitfalls in targeted mutagenesis.
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