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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant HIV-1 gp140 Protein (group M, subtype CRF07_BC) (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:HIV gp140蛋白, HIV gp140 protein
蛋白构建:A DNA sequence encoding the HIV-1 envelope glycoprotein gp160 extracellular domain (Val30-Tyr677), termed as gp140, was fused with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 94 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to biotinylated human CD4-his (Cat:10400-H08H) in functional Elisa.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 30
蛋白分子量:The recombinant HIV-1 gp140 consists of 694 amino acids after removal of the signal peptide and has a predicted molecular mass of 78.2 kDa. The apparent molecular mass of the recombinant protein is approximately 119 KDa in SDS-PAGE under reducing conditions.
蛋白NP号:0
蛋白氨基酸序列:Val30-Tyr677
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions
[资源] 申请上传 Current Protocols In Protein Science (60M) 见公共邮箱
5.7 Overview of Protein Expression in Pichia pastoris Unit 5.8 Culture of Yeast for the Production of Heterologous Proteins Unit 5.9 Overview of Protein Expression by Mammalian Cells Unit 5.10 Production of Recombinant Proteins in Mammalian Cells Unit 5.11
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
., Finstad, J., Williams, Jr., R. C. 1970. Phylogenetic insight into evolution of mammalian Fc fragment of g G globulin using staphylococcal protein A. J. Immunol. 104:140-147. Kronvall, G. 1973. A surface component in group A, C, and G streptococci
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