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100 µg
蛋白名称:SIRP alpha蛋白, SIRP alpha protein
蛋白构建:A DNA sequence encoding the extracellular domain of mouse SIRPA (BAA20376.1) (Met 1-Asn 373) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized rat CD47-His (Cat:80305-R08H) at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Thr 32
蛋白分子量:The secreted recombinant mouse SIRPA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 583 amino acids and has a calculated molecular mass of 64.9 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA/Fc monomer is approximately 1001-20 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:BAA20376.1
蛋白氨基酸序列:Met1-Asn373
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验【求助】矛盾的实验结果:IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达,结合文献,推理应该是IKb alpha下降,而NFKB升高 (IKb可磷酸化NFKB,使后者降低) 但现在我的结果是两者同时升高,请问可能的原因有哪些呢? 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况? erik NFKB是检测核内的吗?检测p65还是p50/ 吴佰霖 检测总蛋白
LABELING ALPHA-ACTININ WITH IATR
.Dialyze overnight against buffer 5 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 12.Determine the concentration of alpha-actinin using Lowry assay.Determine dye/protein molar ratio by diluting conjugate 1:40 (10μl in 400μl
Measurements of DNA Immobilized in the Alpha-Hemolysin Nanopore
In the past decade, there have been extensive studies aimed at exploring the potential of protein nanopores to sequence single strands of DNA using resistive pulse sensing. The high speed of DNA electrophoretically driven through these pores
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