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20 µg/50 µg
| 规格: | 20 µg | 产品价格: | ¥2310 |
|---|---|---|---|
| 规格: | 50 µg | 产品价格: | ¥3870 |
蛋白名称:Factor VII蛋白, Factor VII protein
蛋白构建:A DNA sequence encoding the mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.
表达宿主:CHO Stable Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his (Cat:50413-M08H). The EC50 of biotinylated mouse F3-his (Cat:50413-M08H) is 0.1-0.3 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 25 & Ala 42
蛋白分子量:The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
蛋白NP号:NP_034302.2
蛋白氨基酸序列:Met1-Leu446
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Identification of Mutations in the Human Factor VII Gene
It has been recognized from the early 1800s that activation of coagulation can be initiated by the exposure of subendothelial layers (tissue factor), but it was the 1940s before factor VII (FVII) was included in this event
HIS 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面: 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螫合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲
蛋白标签(protein tag)是指利用DNA体外重组技术,与目的蛋白一起融合表达的一种多肽或者蛋白,以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展,研究人员相继开发出了具有各种不同功能的蛋白标签。 一、 新型蛋白标签 蛋白标签应用极为广泛,但仍有两个问题不容忽视:(1)蛋白标签以DNA形式编码,它需要转录并翻译成蛋白后才能发挥作用,而这种作用方式本身就是一种缺陷。例如,常用的荧光蛋白标签,在显微镜下观察时,其显像不如人工合成的荧光基团好,但是这种人工合成的荧
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