- ￥580 - 8980
- Sino Biological
- 北京
- 11050-HNAC
- 2026年01月23日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human β-NGF / Beta-NGF Protein
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1.00 mg/20.00 µg/100.00 µg/500.00 µg
| 规格： | 1.00 mg | 产品价格： | ￥8980.0 |
|---|---|---|---|
| 规格： | 20.00 µg | 产品价格： | ￥580.0 |
| 规格： | 100.00 µg | 产品价格： | ￥1580.0 |
| 规格： | 500.00 µg | 产品价格： | ￥4480.0 |
蛋白名称：Human β-NGF / Beta-NGF Protein
蛋白构建：A DNA sequence encoding the mature form of human β-NGF (NP_002497.2) (Ser 122-Arg 239) was expressed.
表达宿主：CHO Stable Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ser 122
蛋白分子量：The mature recombinant human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution.
蛋白NP号：NP_002497.2
蛋白氨基酸序列：Ser122-Arg239
蛋白标签：Native
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Zheng, Z; et al. Histone deacetylase 6 inhibition restores autophagic flux to promote functional recovery after spinal cord injury. Experimental neurology, PMID: 31794745
3, Zhou, K; et al. TFE
3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress. Theranostics, PMID: 32802192
4, Liao, S; et al. A bionic multichannel nanofiber conduit carrying Tubastatin A for repairing injured spinal cord. Materials today. Bio, PMID: 36310542
5, Lu, E; et al. Stub1 ameliorates ER stress-induced neural cell apoptosis and promotes locomotor recovery through restoring autophagy flux after spinal cord injury. Experimental neurology, PMID: 37495008
6, Cheng, C; et al. Nerve growth factor promote osteogenic differentiation of dental pulp stem cells through MEK/ERK signalling pathways. Journal of cellular and molecular medicine, PMID: 38333908
7, Fang, Z; et al. Topical TYK2 inhibitor ameliorates psoriasis-like dermatitis via the AKT-SP1-NGFR-AP1 pathway in keratinocytes. Clinical and translational medicine, PMID: 40038877
8, Fang, Z; et al. Topical TYK2 inhibitor ameliorates psoriasis-like dermatitis via the AKT-SP1-NGFR-AP1 pathway in keratinocytes. Clinical and translational medicine, PMID: 40038877
9, Su, H; et al. A self-assembling peptide-based hydrogel containing NF-κB inhibitors and NGF for peripheral nerve injury repair. Biofabrication, PMID: 40145273
10, Yang, J; et al. Nuclear MicroRNA-124-3p Promotes Neurite Outgrowth After Spinal Cord Injury by Enhancing Cttn Transcription. Molecular neurobiology, PMID: 40044957
11, Zou, Z; et al. miR-218a-5p derived from neural stem cell-exosomes inhibits ferroptosis in spinal cord injury through the Bmil/Mettl3/Alox12 axis. International journal of biological macromolecules, PMID: 41067360
Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
to the SAMPLE BUFFER to increase the amount of NGF removed from the sample. Typically, a 10x dilution (w/v) of tissue samples will give a signal sufficient for detection. Once diluted, samples are homogenized on ice for 30 seconds using a cell sonicator at micro
真爱满行囊 我最近在体外细胞做的关于gsk3-beta功能问题，发现药物处理以后，gsk-3-beta的总表达量下调，如果是这样的话 1、我是否能够得出gsk-3-beta的活性下调的结论？ 2、是否还有必要做非活性形式的表达量？我的理解是，基础状态下，gsk-3beta维持的是低活性状态，如果总量都下调了，那应该能够得出活性降低的结论了吧？ 3、另外，在这种情况下我是否有必要去做gsk-3-beta的216位点的活性形式的表达
test 每只大鼠取双侧海马CA3区DG区切片各两张，在计算机图像分析系统（Meta Morph／Ax70）上测量NGF、NT3 平均光密度和平均目标灰度值(所测阳性细胞平均灰度),灰度单位分为256个等级,免疫反应的强弱与灰度值呈反比关系。 采用SPSS for Windows 10.0软件分析，数据以均数加减标准差(x- ±s）表示,组间检验采用t检验,p值取0.05。porsolt swim test (责任编辑：admin)
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