- ￥600 - 1699
- Sino Biological
- 10218-MM01
- 2025年08月12日
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20 µL/100 µL
| 规格： | 20 µL | 产品价格： | ￥600 |
|---|---|---|---|
| 规格： | 100 µL | 产品价格： | ￥1699 |
抗体种属：Human
抗体靶点：CSF3R
抗体应用：ELISA,ELISA(Det)
抗原货号：10218-HCCH
抗原描述：Human cell-derived rhG-CSFR extracellular domain (Catalog#10218-HCCH)
抗体宿主：Mouse
抗体Ig类型：Mouse IgG1
抗体纯化方法：Protein A
抗体制备：This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human G-CSFR extracellular domain (rhG-CSFR; Catalog #10218-HCCH; Met1-Pro621; NP_000751.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
抗体特异性：Human CD114 / G-CSFR
抗体保存：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
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文献和实验Triton X-114 Phase Partitioning for Antigen Characterization
In 1981, Bordier (1 ) first demonstrated that Triton X-114 could be exploited as a means of separating hydrophilic and integral membrane proteins. Since then, Triton X-114 phase partitioning has been extensively used for identification
There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
。加入甘氨酸可以消除甲醛使交联反应终止。1.1.准备两个长满细胞的150cm2的细胞培养皿（1*107-5*107 个细胞/皿）。将甲醛直接滴入PBS洗过的细胞培养皿中，使其终浓度为0.75%，然后在室温缓慢旋转10分钟，使蛋白和DNA发生交联。1.2 加入甘氨酸使其终浓度为125mM,在室温晃动孵育5分钟。1.3 使用10ml预冷PBS清洗细胞2次1.4 使用细胞刮将细胞收获放入 5ml 预冷PBS中，并转入50ml的管子。1.5.在皿里加入3mlPBS，将剩余的细胞转移到50ml管子里1000g
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